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hiscript iii 1st strand cdna synthesis kit  (Vazyme Biotech Co)


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    Vazyme Biotech Co hiscript iii 1st strand cdna synthesis kit
    Hiscript Iii 1st Strand Cdna Synthesis Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 5438 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hiscript+iii+1st+strand+cdna+synthesis+kit/HiScript+III+1st+Strand+cDNA+Synthesis+Kit+%2BgDNA+wiper/pm42892336-221-12-19
    Average 99 stars, based on 5438 article reviews
    hiscript iii 1st strand cdna synthesis kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Rhizosphere Penicillium and phosphorus availability drive bioactive compound accumulation in Amomum villosum.
    Article Snippet: .. RNA was extracted from the roots or leaves of A. villosum using the Fast Pure Plant Total RNA Isolation Kit (Polysaccharides & Polyphenolics-Rich) from Vazyme, followed by reverse transcription into cDNA using the HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) from the same company. .. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis of target genes was then performed: quantitative primers were designed using the Primer-BLAST tool on the NCBI platform, synthesized by Shanghai Sangon Biotech Co., Ltd., and their sequences are detailed in Supplementary Table 9. qRT-PCR reactions were conducted using Vazyme’s ChamQ Universal SYBR qRT-PCR Master Mix.

    Reverse Transcription:

    Article Title: Rhizosphere Penicillium and phosphorus availability drive bioactive compound accumulation in Amomum villosum.
    Article Snippet: .. RNA was extracted from the roots or leaves of A. villosum using the Fast Pure Plant Total RNA Isolation Kit (Polysaccharides & Polyphenolics-Rich) from Vazyme, followed by reverse transcription into cDNA using the HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) from the same company. .. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis of target genes was then performed: quantitative primers were designed using the Primer-BLAST tool on the NCBI platform, synthesized by Shanghai Sangon Biotech Co., Ltd., and their sequences are detailed in Supplementary Table 9. qRT-PCR reactions were conducted using Vazyme’s ChamQ Universal SYBR qRT-PCR Master Mix.

    Article Title: Targeting SUV39H2 exon skipping reverses resistance to CDK4/6 inhibitors via transcriptional activation of p21
    Article Snippet: RNA quality and concentration were detected with a NanoDrop 2000 (Invitrogen). .. RNA was reverse-transcribed to cDNA using HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) (Vazyme, R312-01). .. RT-qPCR was performed with ChamQ Universal SYBR qPCR Master Mix (Vazyme, Q711-02).

    Article Title: African swine fever virus protein R298L opposes the NF-κB signaling pathway and interferon-mediated pathway.
    Article Snippet: African swine fever virus (ASFV) is a large DNA virus that is highly pathogenic to domestic pigs and severely threatens the global pig industry.. ASFV has evolved a range of strategies to evade or suppress the innate antiviral immunity of the host in order to promote its own infection and proliferation.. However, the molecular mechanisms underlying the inhibition of innate antiviral immunity by ASFV-encoded proteins remain unclear.

    cDNA Synthesis:

    Article Title: Rhizosphere Penicillium and phosphorus availability drive bioactive compound accumulation in Amomum villosum.
    Article Snippet: .. RNA was extracted from the roots or leaves of A. villosum using the Fast Pure Plant Total RNA Isolation Kit (Polysaccharides & Polyphenolics-Rich) from Vazyme, followed by reverse transcription into cDNA using the HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) from the same company. .. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis of target genes was then performed: quantitative primers were designed using the Primer-BLAST tool on the NCBI platform, synthesized by Shanghai Sangon Biotech Co., Ltd., and their sequences are detailed in Supplementary Table 9. qRT-PCR reactions were conducted using Vazyme’s ChamQ Universal SYBR qRT-PCR Master Mix.

    Article Title: Targeting SUV39H2 exon skipping reverses resistance to CDK4/6 inhibitors via transcriptional activation of p21
    Article Snippet: RNA quality and concentration were detected with a NanoDrop 2000 (Invitrogen). .. RNA was reverse-transcribed to cDNA using HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) (Vazyme, R312-01). .. RT-qPCR was performed with ChamQ Universal SYBR qPCR Master Mix (Vazyme, Q711-02).

    Article Title: The mitochondrial PPR protein AS3 interacts with NPPR2 to facilitate trans-splicing of nad1 intron 1 and ensure kernel development in maize.
    Article Snippet: In higher plants, many mitochondrial genes contain group II introns that undergo intricate splicing processes to produce mature transcripts, which are subsequently translated into functional proteins.. However, the identities and specific roles of proteins involved in mitochondrial intron splicing in maize remain largely unclear.. In this study, we identified a maize mutant, abnormal seed 3 (as3), through ethyl methanesulfonate (EMS) mutagenesis and mapped the causal gene using bulked segregant analysis.

    Article Title: Shenkang injection ameliorates peritoneal fibrosis via the ESR1/PI3K/Akt pathway: Experimental verification and network pharmacology analysis.
    Article Snippet: Background: Peritoneal fibrosis (PF) is the most distressing complication for peritoneal dialysis (PD) patients, leading to ultrafiltration failure and forcing discontinuation of PD.. However, no effective treatment is currently available.. Shenkang injection (SKI) is a traditional Chinese medicine which has shown antifibrosis efficacy.

    Article Title: A nuclear receptor PcHR96h regulates ABC transporters involved in spirodiclofen resistance in Panonychus citri.
    Article Snippet: Total RNA was extracted using the FastPure Universal Plant Total RNA Isolation Kit (Vazyme, China) following the manufacturer’s instructions. .. First-strand cDNA was synthesized with 1000 ng of total RNA using the HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper; Vazyme). .. Genomic DNA was isolated using the FastPure Cell Tissue DNA Isolation Mini Kit (Vazyme, China).

    Article Title: AcCHI , a Chitinase from Atriplex canescens , Confers Enhanced Tolerance to Osmotic and Salt Stress in Glycine max by Reinforcing Antioxidant and Osmotic Responses.
    Article Snippet: Total RNA was extracted using FreeZol Reagent (Cat. No. R711-01, Vazyme Biotech Co., Ltd., Nanjing, China) according to the manufacturer’s instructions. .. First-strand cDNA was synthesized using the HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) (Vazyme, Nanjing, China; Cat. No. R312-01), and the resulting cDNA was diluted 100-fold for subsequent amplification. .. Quantitative Reverse Transcription PCR was performed using ChamQ Blue Universal SYBR qPCR Master Mix (Cat. No. Q312-02, Vazyme Biotech Co., Ltd., Nanjing, China).

    Article Title: Snf1 promotes lignocellulose degradation by phosphorylating and activating transcription factor Swi6 in Ganoderma lucidum
    Article Snippet: RNA isolation and quantitative reverse transcription PCR (qRT-PCR) Total RNA was isolated with TRIzol reagent (Invitrogen, 15596018) from fungal strains cultured as described above. .. Genomic DNA was removed with DNase I (Vazyme, EN401/402), and cDNA was synthesized using the HiScript III 1st Strand cDNA Synthesis Kit (Vazyme, R312-01). .. qPCR was performed with SupRealQ Purple Universal SYBR qPCR Master Mix (Vazyme, Q412-02) on a Realplex2 Mastercycler EP Gradient S thermocycler (Eppendorf).

    Synthesized:

    Article Title: The mitochondrial PPR protein AS3 interacts with NPPR2 to facilitate trans-splicing of nad1 intron 1 and ensure kernel development in maize.
    Article Snippet: In higher plants, many mitochondrial genes contain group II introns that undergo intricate splicing processes to produce mature transcripts, which are subsequently translated into functional proteins.. However, the identities and specific roles of proteins involved in mitochondrial intron splicing in maize remain largely unclear.. In this study, we identified a maize mutant, abnormal seed 3 (as3), through ethyl methanesulfonate (EMS) mutagenesis and mapped the causal gene using bulked segregant analysis.

    Article Title: Shenkang injection ameliorates peritoneal fibrosis via the ESR1/PI3K/Akt pathway: Experimental verification and network pharmacology analysis.
    Article Snippet: Background: Peritoneal fibrosis (PF) is the most distressing complication for peritoneal dialysis (PD) patients, leading to ultrafiltration failure and forcing discontinuation of PD.. However, no effective treatment is currently available.. Shenkang injection (SKI) is a traditional Chinese medicine which has shown antifibrosis efficacy.

    Article Title: A nuclear receptor PcHR96h regulates ABC transporters involved in spirodiclofen resistance in Panonychus citri.
    Article Snippet: Total RNA was extracted using the FastPure Universal Plant Total RNA Isolation Kit (Vazyme, China) following the manufacturer’s instructions. .. First-strand cDNA was synthesized with 1000 ng of total RNA using the HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper; Vazyme). .. Genomic DNA was isolated using the FastPure Cell Tissue DNA Isolation Mini Kit (Vazyme, China).

    Article Title: AcCHI , a Chitinase from Atriplex canescens , Confers Enhanced Tolerance to Osmotic and Salt Stress in Glycine max by Reinforcing Antioxidant and Osmotic Responses.
    Article Snippet: Total RNA was extracted using FreeZol Reagent (Cat. No. R711-01, Vazyme Biotech Co., Ltd., Nanjing, China) according to the manufacturer’s instructions. .. First-strand cDNA was synthesized using the HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) (Vazyme, Nanjing, China; Cat. No. R312-01), and the resulting cDNA was diluted 100-fold for subsequent amplification. .. Quantitative Reverse Transcription PCR was performed using ChamQ Blue Universal SYBR qPCR Master Mix (Cat. No. Q312-02, Vazyme Biotech Co., Ltd., Nanjing, China).

    Article Title: Snf1 promotes lignocellulose degradation by phosphorylating and activating transcription factor Swi6 in Ganoderma lucidum
    Article Snippet: RNA isolation and quantitative reverse transcription PCR (qRT-PCR) Total RNA was isolated with TRIzol reagent (Invitrogen, 15596018) from fungal strains cultured as described above. .. Genomic DNA was removed with DNase I (Vazyme, EN401/402), and cDNA was synthesized using the HiScript III 1st Strand cDNA Synthesis Kit (Vazyme, R312-01). .. qPCR was performed with SupRealQ Purple Universal SYBR qPCR Master Mix (Vazyme, Q412-02) on a Realplex2 Mastercycler EP Gradient S thermocycler (Eppendorf).

    Random Hexamer:

    Article Title: The mitochondrial PPR protein AS3 interacts with NPPR2 to facilitate trans-splicing of nad1 intron 1 and ensure kernel development in maize.
    Article Snippet: In higher plants, many mitochondrial genes contain group II introns that undergo intricate splicing processes to produce mature transcripts, which are subsequently translated into functional proteins.. However, the identities and specific roles of proteins involved in mitochondrial intron splicing in maize remain largely unclear.. In this study, we identified a maize mutant, abnormal seed 3 (as3), through ethyl methanesulfonate (EMS) mutagenesis and mapped the causal gene using bulked segregant analysis.

    Amplification:

    Article Title: AcCHI , a Chitinase from Atriplex canescens , Confers Enhanced Tolerance to Osmotic and Salt Stress in Glycine max by Reinforcing Antioxidant and Osmotic Responses.
    Article Snippet: Total RNA was extracted using FreeZol Reagent (Cat. No. R711-01, Vazyme Biotech Co., Ltd., Nanjing, China) according to the manufacturer’s instructions. .. First-strand cDNA was synthesized using the HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) (Vazyme, Nanjing, China; Cat. No. R312-01), and the resulting cDNA was diluted 100-fold for subsequent amplification. .. Quantitative Reverse Transcription PCR was performed using ChamQ Blue Universal SYBR qPCR Master Mix (Cat. No. Q312-02, Vazyme Biotech Co., Ltd., Nanjing, China).



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